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WB of HEK293 cell lysates: HEK293 cell lysates were detected with anti-GAPDH antibody (10494-1-AP) labeled with FlexAble CoraLite® 488 Kit ( KFA001, green) and anti-NUDT21 antibody (66335-1-Ig) labeled with FlexAble CoraLite® Plus 750 Kit ( KFA024, red). Immunofluorescence of HeLa: PFA-fixed HeLa cells were stained with anti-TDP43 antibody (10782-2-AP) labeled with FlexAble CoraLite® 488 Kit ( KFA001, green), anti-Tom20 antibody (11802-1-AP) labeled with FlexAble CoraLite® Plus 555 Kit ( KFA002, red), and anti-Lamin B1 antibody (12987-1-AP) labeled with FlexAble CoraLite® Plus 647 Kit ( KFA003, cyan). Immunofluorescence of rat brain tissue: FFPE rat brain tissue sections were stained with anti-NeuN antibody (66836-1-Ig) labeled with FlexAble CoraLite® Plus 647 Kit ( KFA023, magenta), anti-TUBB3 antibody 66375-1-Ig) labeled with FlexAble CoraLite® Plus 555 Kit ( KFA022, orange), and CoraLite®488-conjugated GFAP antibody (CL488-60190, green).
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If you observe leaking, we recommend adding more FlexQuencher to remove unbound FlexLinker, or you can try sequential staining of the labeled antibodies. Other technologies are used for similar purposes as a cookie on other platform where cookies are not available or applicable, such as the advertising ID available on mobile devices. Save hands-on and incubation time by completing labeling of all your antibodies in 10 minutes before your experiment, followed by multiplex staining with all labeled antibodies at the same time.
If there is a certificate we’ve missed out on then it’s probably for a 50 metre swim badge at the local swimming baths. Accidentally" is an adverb derived from the noun "accident," denoting an unintended or unexpected event. Q: Will I observe cross-reactivity/leaking when I use two FlexAble-labeled antibodies from the same species during multiplexing?Common English spelling patterns: In English, words ending in "-ible" or "-able" often have similar meanings, and this is a common spelling pattern.