Roland Cy-5 Dual Trigger Cymbal Pad, 10In

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Kvach, Maksim V.; Ustinov, Alexey V.; Stepanova, Irina A.; Malakhov, Andrei D.; Skorobogatyi, Mikhail V.; Shmanai, Vadim V.; Korshun, Vladimir A. (2008). "A Convenient Synthesis of Cyanine Dyes: Reagents for the Labeling of Biomolecules". European Journal of Organic Chemistry. 2008 (12): 2107–2117. doi: 10.1002/ejoc.200701190. ISSN 1099-0690. Kaur, G., Lewis, J.S. & van Oijen, A.M. Shining a Spotlight on DNA: Single-Molecule Methods to Visualise DNA. Molecules 24 (2019). The amount of Cy5.5 required for reaction depends on the amount of protein to be labeled, and the optimal molar ratio of Cy5.5 to protein is about 10. Pershina, A. G.; Demin, A. M.; Perekucha, N. A.; Brikunova, O. Y.; Efimova, L. V.; Nevskaya, K. V.; Vakhrushev, A. V.; Zgoda, V. G.; Uimin, M. A.; Minin, A. S.; Malkeyeva, D.; Kiseleva, E.; Zima, A. P.; Krasnov, V. P.; Ogorodova, L. M. Peptide Ligands on the PEGylated Nanoparticle Surface and Human Serum Composition Are Key Factors for the Interaction between Immune Cells and Nanoparticles. Colloids and Surfaces B: Biointerfaces, 2023, 221, 112981. doi: 10.1016/j.colsurfb.2022.112981 or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST

Seidel, C.A.M., Schulz, A. & Sauer, M.H.M. Nucleobase-specific quenching of fluorescent dyes .1. Nucleobase one-electron redox potentials and their correlation with static and dynamic quenching efficiencies. J. Phys. Chem.-Us 100, 5541–5553 (1996). Lietard, J., Ameur, D. & Somoza, M. M. Sequence-dependent quenching of fluorescein fluorescence on single-stranded and double-stranded DNA. Rsc Adv. 12, 5629–5637 (2022). Cyanine dyes are used to label proteins, antibodies, peptides, nucleic acid probes, and any kind of other biomolecules to be used in a variety of fluorescence detection techniques: Flow cytometry, Microscopy (mainly Visible range, but also UV, IR), Microplate assays, Microarrays, as well as "light-up Probes," and in vivo imaging. [18] Nucleic acid labeling [ edit ]Cy5.5) = mmol (Cy5.5) ×MW (Cy5.5) / mg/μL (Cy5.5) = 6.7 ×10-5 mmol ×753.88 mg/mmol / 0.01 mg/μL = 5.05 μL (Cy5.5) Huang, Z. X. et al. Spectral identification of specific photophysics of Cy5 by means of ensemble and single molecule measurements. J. Phys. Chem. A 110, 45–50 (2006). structure or technology of the Products, or use the Products for the purpose of developing any products or services that would The word cyanin is from the English word "cyan", which conventionally means a shade of blue-green (close to "aqua") and is derived from the Greek κυάνεος/ κυανοῦς kyaneos/kyanous which means a somewhat different color: "dark blue".

These XGCGC motifs suggest that the identity of the final nucleobase may not be critical for high fluorescence. Indeed, in the 2nd–4th octiles of Cy3/Cy5 fluorescence (Fig. 3A,B), the identity of the second nucleobase appears to be as important as the terminal base and G nucleotides at the penultimate position occupy a large part of the high Cy3 and Cy5 signal intensity. In the second octile of Cy5 fluorescence (Fig. 3B), the 4th position still shows strong G preference, as strong as the 2nd position, which is likely a consequence of the presence of the GCG motif, a trimmed down version of the GCGC that can still enhance the fluorescence properties of Cy5, though less so for Cy3. Umezawa K, Matsui A, Nakamura Y, Citterio D, Suzuki K (2009). "Bright, color-tunable fluorescent dyes in the Vis/NIR region: establishment of new "tailor-made" multicolor fluorophores based on borondipyrromethene". Chemistry: A European Journal. 15 (5): 1096–106. doi: 10.1002/chem.200801906. PMID 19117043. Perez-Gonzalez, C., Lafontaine, D.A. & Penedo, J.C. Fluorescence-Based Strategies to Investigate the Structure and Dynamics of Aptamer-Ligand Complexes. Front. Chem. 4 (2016). Holz, K., Lietard, J. & Somoza, M. M. High-power 365 nm UV LED mercury arc lamp replacement for photochemistry and chemical photolithography. ACS Sustain. Chem. Eng. 5, 828–834 (2017).

This Cyanine5 NHS ester (analog to Cy5 ® NHS ester) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires a small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reactions (please see our recommended protocol for more details). This reagent is ideal for very cost-efficient labeling of soluble proteins as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. For more sophisticated targets such as easily degradable proteins, when the use of DMF or DMSO is undesirable, consider using water-soluble sulfo-Cyanine 5 NHS ester which does not require any co-solvent, and features very similar fluorescent properties. Cyanine dyes are available with different modifications such as methyl, ethyl or butyl substituents, carboxyl, acetylmethoxy, and sulfo groups which alter their hydrophilicity. [8] Probe Cy3 and Cy5 are used in proteomics experiments so that samples from two sources can be mixed and run together through the separation process. [19] [20] This eliminates variations due to differing experimental conditions that are inevitable if the samples were run separately. These variations make it extremely difficult, if not impossible, to use computers to automate the acquisition of the data after the separation is complete. Using these dyes makes the automation trivial. Unlü M, Morgan ME, Minden JS (Oct 1997). "Difference gel electrophoresis: a single gel method for detecting changes in protein extracts". Electrophoresis. 18 (11): 2071–7. doi: 10.1002/elps.1150181133. PMID 9420172. S2CID 604007.

Holz, K., Schaudy, E., Lietard, J. & Somoza, M. M. Multi-level patterning nucleic acid photolithography. Nat. Commun. 10, 3805 (2019).Depends strongly on viscosity, temperature, and biomolecular interactions. [11] Common cyanine dyes and their uses [ edit ] Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any Zhu, H.L., Fohlerova, Z., Pekarek, J., Basova, E. & Neuzil, P. Recent advances in lab-on-a-chip technologies for viral diagnosis. Biosens. Bioelectron. 153 (2020). Cy5 is sensitive to its electronic environment. Changes in the conformation of the protein it is attached to will produce either enhancement or quenching of the emission. The rate of this change can be measured to determine enzyme kinetic parameters. The dyes can be used for similar purposes in FRET experiments.

For protein labeling, Cy3 and Cy5 dyes sometimes bear a succinimidyl group to react with amines, or a maleimide group to react with a sulfhydryl group of cysteine residues. Kretschy, N., Holik, A. K., Somoza, V., Stengele, K. P. & Somoza, M. M. Next-generation o-nitrobenzyl photolabile groups for light-directed chemistry and microarray synthesis. Angew. Chem. Int. Ed. 54, 8555–8559 (2015). Agbavwe, C. & Somoza, M. M. Sequence-dependent fluorescence of cyanine dyes on microarrays. PLoS ONE 6, e22177 (2011). Kim, Eunha; Park, Seung Bum (2010). "Discovery of New Synthetic Dyes: Targeted Synthesis or Combinatorial Approach?". In Demchenko, Alexander P. (ed.). Advanced Fluorescence Reporters in Chemistry and Biology I: Fundamentals and Molecular Design Volume 8 of Springer Series on Fluorescence. Berlin: Springer. p.172. ISBN 9783642047022.Cy3B: This product is manufactured under an exclusive license from Carnegie Mellon University and is covered by US patent number 6,133,445 and equivalent patents and patent applications in other countries. CytoCy5S: The use of this product in an NTR gene reporter assay is the subject of US patent number 7,579,140 in the name of Cytiva. Cy7 is a near-IR fluor that is invisible to the naked eye (excitation/emission maximum 750/776nm). It is used in in vivo imaging applications, as well as the Cy7.5 dye.